Differentiation of Dictyostelium discoideum Cells in Suspension Culture
نویسندگان
چکیده
منابع مشابه
Differentiation of Dictyostelium discoideum mutant cells in a shaken suspension culture and the effect of cyclic AMP.
In Dictyostelium discoideum, 16 mutants in which cells differentiate into spores and stalk cells without normal morphogenesis were isolated. All these mutants are rapidly developing and capable of differentiating in a shaken suspension of phosphate buffer. The developmental kinetics of specific activities of enzymes in one of the mutants, HTY 1851, cultured in the suspension was compared with t...
متن کاملDictyostelium discoideum during Growth in Axenic Culture
1. The specific activities of fl-N-acetylglucosaminidase, acid phosphatase, ac-mannosidase, P-glucosidase, UDP-glucose pyrophosphorylase and alkaline phosphatase have been determined in myxamoebae of the cellular slime mould Dictyostelium discoideum Ax-2 grown on different media and in different phases of the growth cycle. 2. Variations in enzymic composition occur with changes in growth medium...
متن کاملTrehalose Synthesis during Differentiation in Dictyostelium discoideum
The synthesis of [%Z]trehalose has been studied in uiuo at various stages of differentiation in Dictyostelium discoideum. The rate of synthesis was negligible from aggregation until late in the culmination process. At that time, a lOO-fold burst of synthetic activity was observed, amounting to 0.04 pmole of trehalose per min per ml of packed cells. In the course of these studies radioactive mal...
متن کاملTrehalose Synthesis during Differentiation in Dictyostelium discoideum
The synthesis of [%Z]trehalose has been studied in uiuo at various stages of differentiation in Dictyostelium discoideum. The rate of synthesis was negligible from aggregation until late in the culmination process. At that time, a lOO-fold burst of synthetic activity was observed, amounting to 0.04 pmole of trehalose per min per ml of packed cells. In the course of these studies radioactive mal...
متن کاملInter-relation of cell adhesion and differentiation in Dictyostelium discoideum.
During the development of Dictyostelium discoideum single cells aggregate to form a multicellular organism. The change from the single cell state to multicellularity is mediated by chemotactic cell movement and intercellular adhesion. Dictyostelium represents a side branch in the evolution of multicellular organization in eukaryotes. Therefore, the general importance of any work done in Dictyos...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1981
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-127-2-301